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Prevalence of Very Low Numbers of Potential Pathogenic Isolates of Yersiniaenterocolitica and Yersinia intermedia in Traditional Fast Foods of India.

ABSTRACT: In this study, an incidence pattern of 1.7% for Yersiniaenterocolitica and 2.5% for Y. intermedia were observed in an analysis of 120 diversified food samples collected from the local market of Mysore, Southern India. Two native isolates characterized as Y. enterocolitica belonged to biotype 1B and revealed the presence of major virulence related traits such as regulator of virulence, mucoid Yersinia factor regulator, attachment invasion locus, heat stable enterotoxin, Yersinia type II secretory system and phospholipase A in PCR. Force type neighbor-joining phylograms generated for Y. enterocolitica based on PCR amplicons of rovA and ypl showed 100% homology with two to three strains of Y. enterocolitica and about 75% homology with several strains of Y. pestis.

SUBMITTER: Divya KH

PROVIDER: S-EPMC3209932 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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Prevalence of Very Low Numbers of Potential Pathogenic Isolates of Yersiniaenterocolitica and Yersinia intermedia in Traditional Fast Foods of India.

Divya K H KH Varadaraj M C MC

Indian journal of microbiology 20110422 4

In this study, an incidence pattern of 1.7% for Yersiniaenterocolitica and 2.5% for Y. intermedia were observed in an analysis of 120 diversified food samples collected from the local market of Mysore, Southern India. Two native isolates characterized as Y. enterocolitica belonged to biotype 1B and revealed the presence of major virulence related traits such as regulator of virulence, mucoid Yersinia factor regulator, attachment invasion locus, heat stable enterotoxin, Yersinia type II secretory ...[more]

PMID: 23024408

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Project description:BackgroundThe yersiniae (Enterobacteriaceae) occupy a variety of niches, including some in human and flea hosts. Metabolic adaptations of the yersiniae, which contribute to their success in these specialized environments, remain largely unknown. We report results of an investigation of the transcriptome under aerobic and anaerobic conditions for Y. intermedia, a non-pathogenic member of the genus that has been used as a research surrogate for Y. pestis. Y. intermedia shares characteristics of pathogenic yersiniae, but is not known to cause disease in humans. Oxygen restriction is an important environmental stimulus experienced by many bacteria during their life-cycles and greatly influences their survival in specific environments. How oxygen availability affects physiology in the yersiniae is of importance in their life cycles but has not been extensively characterized.Methodology/principal findingsTiled oligonucleotide arrays based on a draft genome sequence of Y. intermedia were used in transcript profiling experiments to identify genes that change expression in response to oxygen availability during growth in minimal media with glucose. The expression of more than 400 genes, constituting about 10% of the genome, was significantly altered due to oxygen-limitation in early log phase under these conditions. Broad functional categorization indicated that, in addition to genes involved in central metabolism, genes involved in adaptation to stress and genes likely involved with host interactions were affected by oxygen-availability. Notable among these, were genes encoding functions for motility, chemotaxis and biosynthesis of cobalamin, which were up-regulated and those for iron/heme utilization, methionine metabolism and urease, which were down-regulated.Conclusions/significanceThis is the first transcriptome analysis of a non-pathogenic Yersinia spp. and one of few elucidating the global response to oxygen limitation for any of the yersiniae. Thus this study lays the foundation for further experimental characterization of oxygen-responsive genes and pathways in this ecologically diverse genus.

Dataset Information

Prevalence of Very Low Numbers of Potential Pathogenic Isolates of Yersiniaenterocolitica and Yersinia intermedia in Traditional Fast Foods of India.

Publications

Prevalence of Very Low Numbers of Potential Pathogenic Isolates of Yersiniaenterocolitica and Yersinia intermedia in Traditional Fast Foods of India.

Similar Datasets

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