Ontology highlight
ABSTRACT:
SUBMITTER: Do K
PROVIDER: S-EPMC3212612 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20111024 45
Truncated green fluorescent protein (GFP) that is refolded after removing the 10th β-strand can readily bind to a synthetic strand to recover the absorbance and fluorescence of the whole protein. This allows rigorous experimental determination of thermodynamic and kinetic parameters of the split system including the equilibrium constant and the association/dissociation rates, which enables residue-specific analysis of peptide-protein interactions. The dissociation rate of the noncovalently bound ...[more]