Ontology highlight
ABSTRACT:
SUBMITTER: Martinez-Rodriguez S
PROVIDER: S-EPMC321266 | biostudies-literature | 2004 Jan
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20040101 1
Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins with short rather than long aliphatic side chains or those with aromatic rings. Substrates, which showed no detectable activity toward the enzyme, were found to exhibit competitive i ...[more]