Ontology highlight
ABSTRACT:
SUBMITTER: Okabe Y
PROVIDER: S-EPMC3212723 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Okabe Yoshihiro Y Asamizu Erika E Saito Takeshi T Matsukura Chiaki C Ariizumi Tohru T Brès Cécile C Rothan Christophe C Mizoguchi Tsuyoshi T Ezura Hiroshi H
Plant & cell physiology 20110930 11
To accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screening 10 genes. The 0.5% EMS population had a mild mutation frequency of one mutation per 1,710 kb, whereas the 1.0% EMS population had a frequency of one mutation per 737 kb, a frequency suitable for pro ...[more]