Ontology highlight
ABSTRACT:
SUBMITTER: Savan R
PROVIDER: S-EPMC321279 | biostudies-literature | 2004 Jan
REPOSITORIES: biostudies-literature
Savan Ram R Igarashi Arisa A Matsuoka Satoru S Sakai Masahiro M
Applied and environmental microbiology 20040101 1
Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65 degrees C. No amplification of the target hemolysin gene was detected in other related bacteria. When the LAMP primers were used, detection of edwardsiellosis in infec ...[more]