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Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.

ABSTRACT: A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could effectively differentiate B. caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection.

SUBMITTER: Tamaki Y

PROVIDER: S-EPMC321337 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature

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Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.

Tamaki Yoh Y Hirata Haruyuki H Takabatake Noriyuki N Bork Sabine S Yokoyama Naoaki N Xuan Xuenan X Fujisaki Kozo K Igarashi Ikuo I

Clinical and diagnostic laboratory immunology 20040101 1

A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could effectively differentiate B. caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection. ...[more]

PMID: 14715570

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