Light-initiated hydroxylation of lauric acid using hybrid P450 BM3 enzymes.
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ABSTRACT:
SUBMITTER: Tran NH
PROVIDER: S-EPMC3213755 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
Light-initiated hydroxylation of lauric acid using hybrid P450 BM3 enzymes.
Tran Ngoc-Han NH Huynh Ngoc N Bui Thuba T Nguyen Yen Y Huynh Phuong P Cooper Mary E ME Cheruzel Lionel E LE
Chemical communications (Cambridge, England) 20111005 43
We have developed hybrid P450 BM3 enzymes consisting of a Ru(II)-diimine photosensitizer covalently attached to non-native single cysteine residues of P450 BM3 heme domain mutants. These enzymes are capable, upon light activation, of selectively hydroxylating lauric acid with 40 times higher total turnover numbers compared to the peroxide shunt. ...[more]
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