Questioning the functional relevance of mitochondrial supercomplexes by time-resolved analysis of the respiratory chain.

ABSTRACT: Mitochondria are the powerhouses of eukaryotic cells as they feed metabolism with its major substrate. Oxidative-phosphorylation relies on the generation, by an electron/proton transfer chain, of an electrochemical transmembrane potential utilized to synthesize ATP. Although these fundamental principles are not a matter of debate, the emerging picture of the respiratory chain diverges from the linear and fluid scheme. Indeed, a growing number of pieces of evidence point to membrane compartments that possibly restrict the diffusion of electron carriers, and to supramolecular assembly of various complexes within various kinds of supercomplexes that modulate the thermodynamic and kinetic properties of the components of the chain. Here, we describe a method that allows the unprecedented time-r

SUBMITTER: Trouillard M 

PROVIDER: S-EPMC3215061 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature