Cd8 enhancer E8I and Runx factors regulate CD8? expression in activated CD8+ T cells.

ABSTRACT: Cd8a and Cd8b1 coreceptor gene (Cd8) expression is tightly controlled during T-cell development by the activity of five Cd8 enhancers (E8(I)-E8(V)). Here we demonstrate a unique transcriptional program regulating CD8 expression during CD8(+) effector T-cell differentiation. The Cd8 enhancer E8(I) and Runx/core-binding factor-? (CBF?) complexes were required for the establishment of this regulatory circuit, because E8(I)-, Runx3-, or CBF?-deficient CD8(+) T cells down-regulated CD8? expression during activation. This finding correlated with enhanced repressive histone marks at the Cd8a promoter in the absence of E8(I), and the down-regulation of CD8? expression could be blocked by treating E8(I)-, Runx3-, or CBF?-deficient CD8(+) T cells with the histone deacetylase inhibitor trichostatin A. Moreover, Runx/CBF? complexes bound the Cd8ab gene cluster in activated CD8(+) T cells, suggesting direct control of the Cd8a locus. However, CD8(+) effector T cells maintained high levels of CD8? when CBF? was conditionally deleted after activation. Thus, our data suggest an E8(I)- and Runx3/CBF?-dependent epigenetic programming of the Cd8a locus during T-cell activation, leading to Runx/CBF? complex-independent maintenance of CD8? expression in effector T cells.

SUBMITTER: Hassan H 

PROVIDER: S-EPMC3215065 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature