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Altered branching patterns of Purkinje cells in mouse model for cortical development disorder.


ABSTRACT: Disrupted cortical cytoarchitecture in cerebellum is a typical pathology in reeler. Particularly interesting are structural problems at the cellular level: dendritic morphology has important functional implication in signal processing. Here we describe a combinatorial imaging method of synchrotron X-ray microtomography with Golgi staining, which can deliver 3-dimensional(3-D) micro-architectures of Purkinje cell(PC) dendrites, and give access to quantitative information in 3-D geometry. In reeler, we visualized in 3-D geometry the shape alterations of planar PC dendrites (i.e., abnormal 3-D arborization). Despite these alterations, the 3-D quantitative analysis of the branching patterns showed no significant changes of the 77 ± 8° branch angle, whereas the branch segment length strongly increased with large fluctuations, comparing to control. The 3-D fractal dimension of the PCs decreased from 1.723 to 1.254, indicating a significant reduction of dendritic complexity. This study provides insights into etiologies and further potential treatment options for lissencephaly and various neurodevelopmental disorders.

SUBMITTER: Kim J 

PROVIDER: S-EPMC3216603 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Altered branching patterns of Purkinje cells in mouse model for cortical development disorder.

Kim Jinkyung J   Kwon Namseop N   Chang Soeun S   Kim Kyong-Tai KT   Lee Dongmyeong D   Kim Seunghwan S   Yun So Jeong SJ   Hwang Daehee D   Kim Jee Woong JW   Hwu Yeukuang Y   Margaritondo Giorgio G   Je Jung Ho JH   Rhyu Im Joo IJ  

Scientific reports 20111018


Disrupted cortical cytoarchitecture in cerebellum is a typical pathology in reeler. Particularly interesting are structural problems at the cellular level: dendritic morphology has important functional implication in signal processing. Here we describe a combinatorial imaging method of synchrotron X-ray microtomography with Golgi staining, which can deliver 3-dimensional(3-D) micro-architectures of Purkinje cell(PC) dendrites, and give access to quantitative information in 3-D geometry. In reele  ...[more]

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