Ontology highlight
ABSTRACT:
SUBMITTER: McIsaac RS
PROVIDER: S-EPMC3216669 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
McIsaac R Scott RS Silverman Sanford J SJ McClean Megan N MN Gibney Patrick A PA Macinskas Joanna J Hickman Mark J MJ Petti Allegra A AA Botstein David D
Molecular biology of the cell 20110930 22
We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the hormone β-estradiol, cytoplasmic GEV localizes to the nucleus and binds to promoters containing Gal4p consensus binding sequences to activate transcription. With galactokinase Gal1p and ...[more]