Characterization of the Na?/H? antiporter from Yersinia pestis.
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ABSTRACT:
SUBMITTER: Ganoth A
PROVIDER: S-EPMC3216949 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
Ganoth Assaf A Alhadeff Raphael R Kohen Dovrat D Arkin Isaiah T IT
PloS one 20111115 11
Yersinia pestis, the bacterium that historically accounts for the Black Death epidemics, has nowadays gained new attention as a possible biological warfare agent. In this study, its Na⁺/H⁺ antiporter is investigated for the first time, by a combination of experimental and computational methodologies. We determined the protein's substrate specificity and pH dependence by fluorescence measurements in everted membrane vesicles. Subsequently, we constructed a model of the protein's structure and val ...[more]
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