Ontology highlight
ABSTRACT:
SUBMITTER: Hosokawa Y
PROVIDER: S-EPMC3218030 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Hosokawa Yoichiroh Y Ochi Haruki H Iino Takanori T Hiraoka Akihiro A Tanaka Mikiko M
PloS one 20111116 11
Introduction of biomolecules into cells in living animals is one of the most important techniques in molecular and developmental biology research, and has potentially broad biomedical implications. Here we report that biomolecules can be introduced into single cells in living vertebrate embryos by photoporation using a femtosecond laser amplifier with a high pulse energy and a low repetition rate. First, we confirmed the efficiency of this photoporation technique by introducing dextran, morpholi ...[more]