Unexpected instability of family of repeats (FR), the critical cis-acting sequence required for EBV latent infection, in EBV-BAC systems.
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ABSTRACT:
SUBMITTER: Kanda T
PROVIDER: S-EPMC3218044 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Unexpected instability of family of repeats (FR), the critical cis-acting sequence required for EBV latent infection, in EBV-BAC systems.
Kanda Teru T Shibata Sachiko S Saito Satoru S Murata Takayuki T Isomura Hiroki H Yoshiyama Hironori H Takada Kenzo K Tsurumi Tatsuya T
PloS one 20111116 11
A group of repetitive sequences, known as the Family of Repeats (FR), is a critical cis-acting sequence required for EBV latent infection. The FR sequences are heterogeneous among EBV strains, and they are sometimes subject to partial deletion when subcloned in E. coli-based cloning vectors. However, the FR stability in EBV-BAC (bacterial artificial chromosome) system has never been investigated. We found that the full length FR of the Akata strain EBV was not stably maintained in a BAC vector. ...[more]
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