Ontology highlight
ABSTRACT:
SUBMITTER: Tomonaga Y
PROVIDER: S-EPMC3218342 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Tomonaga Yuya Y Hidaka Tetsurou T Kawamura Izuru I Nishio Takudo T Ohsawa Kazuhiro K Okitsu Takashi T Wada Akimori A Sudo Yuki Y Kamo Naoki N Ramamoorthy Ayyalusamy A Naito Akira A
Biophysical journal 20111115 10
A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The (13)C NMR signals from [20-(13)C]retinal-ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuo ...[more]