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An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy.


ABSTRACT: A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The (13)C NMR signals from [20-(13)C]retinal-ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.

SUBMITTER: Tomonaga Y 

PROVIDER: S-EPMC3218342 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy.

Tomonaga Yuya Y   Hidaka Tetsurou T   Kawamura Izuru I   Nishio Takudo T   Ohsawa Kazuhiro K   Okitsu Takashi T   Wada Akimori A   Sudo Yuki Y   Kamo Naoki N   Ramamoorthy Ayyalusamy A   Naito Akira A  

Biophysical journal 20111115 10


A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The (13)C NMR signals from [20-(13)C]retinal-ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuo  ...[more]

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