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Detection of foot-and-mouth disease virus RNA by reverse transcription loop-mediated isothermal amplification.


ABSTRACT: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus. Furthermore, the assay correctly detected 84 FMDV positive samples but not 65 FMDV negative specimens. The result indicated the potential usefulness of the technique as a simple and rapid procedure for the detection of FMDV infection.

SUBMITTER: Chen HT 

PROVIDER: S-EPMC3222687 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Detection of foot-and-mouth disease virus RNA by reverse transcription loop-mediated isothermal amplification.

Chen Hao-tai HT   Zhang Jie J   Liu Yong-sheng YS   Liu Xiang-tao XT  

Virology journal 20111109


A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japa  ...[more]

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