Ontology highlight
ABSTRACT:
SUBMITTER: Chen HT
PROVIDER: S-EPMC3222687 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Chen Hao-tai HT Zhang Jie J Liu Yong-sheng YS Liu Xiang-tao XT
Virology journal 20111109
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japa ...[more]