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The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.

ABSTRACT: SHQ1 is an essential assembly factor for H/ACA ribonucleoproteins (RNPs) required for ribosome biogenesis, pre-mRNA splicing, and telomere maintenance. SHQ1 binds dyskerin/NAP57, the catalytic subunit of human H/ACA RNPs, and this interaction is modulated by mutations causing X-linked dyskeratosis congenita. We report the crystal structure of the C-terminal domain of yeast SHQ1, Shq1p, and its complex with yeast dyskerin/NAP57, Cbf5p, lacking its catalytic domain. The C-terminal domain of Shq1p interacts with the RNA-binding domain of Cbf5p and, through structural mimicry, uses the RNA-protein-binding sites to achieve a specific protein-protein interface. We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins.

SUBMITTER: Walbott H

PROVIDER: S-EPMC3222905 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.

Walbott Hélène H Machado-Pinilla Rosario R Liger Dominique D Blaud Magali M Réty Stéphane S Grozdanov Petar N PN Godin Kate K van Tilbeurgh Herman H Varani Gabriele G Meier U Thomas UT Leulliot Nicolas N

Genes & development 20111101 22

SHQ1 is an essential assembly factor for H/ACA ribonucleoproteins (RNPs) required for ribosome biogenesis, pre-mRNA splicing, and telomere maintenance. SHQ1 binds dyskerin/NAP57, the catalytic subunit of human H/ACA RNPs, and this interaction is modulated by mutations causing X-linked dyskeratosis congenita. We report the crystal structure of the C-terminal domain of yeast SHQ1, Shq1p, and its complex with yeast dyskerin/NAP57, Cbf5p, lacking its catalytic domain. The C-terminal domain of Shq1p ...[more]

PMID: 22085966

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