Dataset Information

A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].

ABSTRACT: Here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. Based on the predicted functions of these identified plastid proteins, a large proportion (?60%) of the chromoplast proteome of sweet orange is constituted by proteins involved in carbohydrate metabolism, amino acid/protein synthesis, and secondary metabolism. Of note, HDS (hydroxymethylbutenyl 4-diphosphate synthase), PAP (plastid-lipid-associated protein), and psHSPs (plastid small heat shock proteins) involved in the synthesis or storage of carotenoid and stress response are among the most abundant proteins identified. A comparison of chromoplast proteomes between sweet orange and tomato suggested a high level of conservation in a broad range of metabolic pathways. However, the citrus chromoplast was characterized by more extensive carotenoid synthesis, extensive amino acid synthesis without nitrogen assimilation, and evidence for lipid metabolism concerning jasmonic acid synthesis. In conclusion, this study provides an insight into the major metabolic pathways as well as some unique characteristics of the sweet orange chromoplasts at the whole proteome level.

SUBMITTER: Zeng Y

PROVIDER: S-EPMC3223033 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].

Zeng Yunliu Y Pan Zhiyong Z Ding Yuduan Y Zhu Andan A Cao Hongbo H Xu Qiang Q Deng Xiuxin X

Journal of experimental botany 20110812 15

Here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. Based on the predicted functions of these identified plastid proteins, a large propo ...[more]

PMID: 21841170

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Project description:Anthocyanins are a class of natural pigments that accumulate transiently or permanently in plant tissues, often in response to abiotic and biotic stresses. They play a photoprotective role by attenuating the irradiance incident on the photochemical apparatus and quenching oxyradicals through their powerful anti-oxidative function. The objective of the current study is to understand the impact of introducing Vitis vinifera mybA1 (VvmybA1) in 'Hamlin' sweet orange trees on various aspects, including photosynthetic performance, pigment composition, and gene expression related to photosynthesis and light harvesting. We describe the relationship between anthocyanin accumulation and photosynthetic measurements in genetically modified 'Hamlin' sweet orange trees expressing the grapevine-derived Vitis vinifera mybA1 (VvmybA1). The juvenile leaves of transgenic plants displayed an intense purple color compared to the mature leaves, and microscopic visualization showed anthocyanin accumulation primarily in the leaf epidermal cells. Under optimal growth conditions, there were no significant differences in leaf gas exchange variables, suggesting normal photosynthetic performance. The chlorophyll fluorescence maximum quantum yield of PSII was slightly reduced in VvmybA1 transgenic leaves compared to the performance of the control leaves, while the total performance index per absorbance remained unaffected. Comparison of the chlorophyll and carotenoid pigment contents revealed that chlorophyllide a and carotenoid pigments, including trans-neoxanthin, trans-violaxanthin, cis-violaxanthin, zeaxanthin, antheraxanthin, and total xanthophylls were enhanced in VvmybA1 transgenic leaves. Although there were no significant changes in the rates of the gas exchange parameters, we recorded a high relative expression of the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RuBP) and rubisco activase (RCA) in the mature leaves of transgenic plants, indicating activation of Rubisco. Our findings confirm an efficient photoacclimation of the photosynthetic apparatus, allowing the transgenic line to maintain a photosynthetic performance similar to that of the wild type.

Dataset Information

A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].

Publications

A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].

Similar Datasets

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