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Regional expression of P2Y(4) receptors in the rat central nervous system.

ABSTRACT: P2Y receptors are G protein-coupled receptors composed of eight known subunits (P2Y(1, 2, 4, 6, 11, 12, 13, 14)), which are involved in different functions in neural tissue. The present study investigates the expression pattern of P2Y(4) receptors in the rat central nervous system (CNS) using immunohistochemistry and in situ hybridization. The specificity of the immunostaining has been verified by preabsorption, Western blot, and combined use of immunohistochemistry and in situ hybridization. Neurons expressing P2Y(4) receptors were distributed widely in the rat CNS. Heavy P2Y(4) receptor immunostaining was observed in the magnocellular neuroendocrine neurons of the hypothalamus, red nucleus, pontine nuclei, mesencephalic trigeminal nucleus, motor trigeminal nucleus, ambiguous nucleus, inferior olive, hypoglossal nucleus, and dorsal motor vagus nucleus. Both neurons and astrocytes express P2Y(4) receptors. P2Y(4) receptor immunostaining signals were mainly confined to cell bodies and dendrites of neurons, suggesting that P2Y(4) receptors are mainly involved in regulating postsynaptic events. In the hypothalamus, all the vasopressin (VP) and oxytocin (OT) neurons and all the orexin A neurons were immunoreactive for P2Y(4) receptors. All the neurons expressing P2Y(4) receptors were found to express N-methyl-D: -aspartate receptor 1 (NR1). These data suggest that purines and pyrimidines might be involved in regulation of the release of the neuropeptides VP, OT, and orexin in the rat hypothalamus via P2Y(4) receptors. Further, the physiological and pathophysiological functions of the neurons may operate through coupling between P2Y(4) receptors and NR1.

SUBMITTER: Song X

PROVIDER: S-EPMC3224639 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Regional expression of P2Y(4) receptors in the rat central nervous system.

Song Xianmin X Guo Wei W Yu Qiang Q Liu Xiaofeng X Xiang Zhenghua Z He Cheng C Burnstock Geoffrey G

Purinergic signalling 20110719 4

P2Y receptors are G protein-coupled receptors composed of eight known subunits (P2Y(1, 2, 4, 6, 11, 12, 13, 14)), which are involved in different functions in neural tissue. The present study investigates the expression pattern of P2Y(4) receptors in the rat central nervous system (CNS) using immunohistochemistry and in situ hybridization. The specificity of the immunostaining has been verified by preabsorption, Western blot, and combined use of immunohistochemistry and in situ hybridization. Ne ...[more]

PMID: 21769584

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Project description:BACKGROUND: Vertebrate classic cadherins are divided into type I and type II subtypes, which are individually expressed in brain subdivisions (e.g., prosomeres, rhombomeres, and progenitor domains) and in specific neuronal circuits in region-specific manners. We reported previously the expression of cadherin19 (cad19) in Schwann cell precursors. Cad19 is a type II classic cadherin closely clustered on a chromosome with cad7 and cad20. The expression patterns of cad7 and cad20 have been reported previously in chick embryo but not in the developing and adult central nervous system of mammals. In this study, we identified rat cad7 and cad20 and analyzed their expression patterns in embryonic and adult rat brains. RESULTS: Rat cad7 protein showed 92% similarity to chick cad7, while rat cad20 protein had 76% similarity to Xenopus F-cadherin. Rat cad7 mRNA was initially expressed in the anterior neural plate including presumptive forebrain and midbrain regions, and then accumulated in cells of the dorsal neural tube and in rhombomere boundary cells of the hindbrain. Expression of rat cad20 mRNA was specifically localized in the anterior neural region and rhombomere 2 in the early neural plate, and later in longitudinally defined ventral cells of the hindbrain. The expression boundaries of cad7 and cad20 corresponded to those of region-specific transcription factors such as Six3, Irx3 and Otx2 in the neural plate, and Dbx2 and Gsh1 in the hindbrain. At later stages, the expression of cad7 and cad20 disappeared from neuroepithelial cells in the hindbrain, and was almost restricted to postmitotic cells, e.g. somatic motor neurons and precerebellar neurons. These results emphasized the diversity of cad7 and cad20 expression patterns in different vertebrate species, i.e. birds and rodents. CONCLUSION: Taken together, our findings suggest that the expression of cad7 and cad20 demarcates the compartments, boundaries, progenitor domains, specific nuclei and specific neural circuits during mammalian brain development.

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Regional expression of P2Y(4) receptors in the rat central nervous system.

Publications

Regional expression of P2Y(4) receptors in the rat central nervous system.

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