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Exploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice.

ABSTRACT: BACKGROUND: Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and development. RESULTS: To determine the putative transcriptional regulation mechanism of OsWRKY13, the putative cis-acting elements of OsWRKY13-influenced genes were analyzed using the whole genome expression profiling of OsWRKY13-activated plants generated with the Affymetrix GeneChip Rice Genome Array. At least 39 transcription factor genes were influenced by OsWRKY13, and 30 of them were downregulated. The promoters of OsWRKY13-upregulated genes were overrepresented with W-boxes for WRKY protein binding, whereas the promoters of OsWRKY13-downregulated genes were enriched with cis-elements putatively for binding of MYB and AP2/EREBP types of transcription factors. Consistent with the distinctive distribution of these cis-elements in up- and downregulated genes, nine WRKY genes were influenced by OsWRKY13 and the promoters of five of them were bound by OsWRKY13 in vitro; all seven differentially expressed AP2/EREBP genes and six of the seven differentially expressed MYB genes were suppressed by in OsWRKY13-activated plants. A subset of OsWRKY13-influenced WRKY genes were involved in host-pathogen interactions. CONCLUSION: These results suggest that OsWRKY13-mediated signalling pathways are partitioned by different transcription factors. WRKY proteins may play important roles in the monitoring of OsWRKY13-upregulated genes and genes involved in pathogen-induced defence responses, whereas MYB and AP2/EREBP proteins may contribute most to the control of OsWRKY13-downregulated genes.

SUBMITTER: Qiu D

PROVIDER: S-EPMC3224702 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Exploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice.

Qiu Deyun D Xiao Jun J Xie Weibo W Cheng Hongtao H Li Xianghua X Wang Shiping S

BMC plant biology 20090618

<h4>Background</h4>Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and development.<h4>Results</h4>To determine the putative transcriptional regulation mechanism of OsWRKY13, the putative cis-acting elements of OsWRKY13-influenced genes were analyzed using the whole genome expression profiling of OsWRKY13-activated plants generated with the Affym ...[more]

PMID: 19534828

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Dataset Information

Exploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice.

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Exploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice.

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