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Mitochondrial electron transport chain complex III is required for antimycin A to inhibit autophagy.

ABSTRACT: Autophagy is a cellular lysosome-dependent catabolic mechanism mediating the turnover of intracellular organelles and long-lived proteins. We show that antimycin A, a known inhibitor of mETC complex III, can inhibit autophagy. A structural and functional study shows that four close analogs of antimycin A that have no effect on mitochondria inhibition also do not inhibit autophagy, whereas myxothiazol, another mETC complex III inhibitor with unrelated structure to antimycin A, inhibits autophagy. Additionally, antimycin A and myxothiazol cannot inhibit autophagy in mtDNA-depleted H4 and mtDNA-depleted HeLa cells. These data suggest that antimycin A inhibits autophagy through its inhibitory activity on mETC complex III. Our data suggest that mETC complex III may have a role in mediating autophagy induction.

SUBMITTER: Ma X

PROVIDER: S-EPMC3225892 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Mitochondrial electron transport chain complex III is required for antimycin A to inhibit autophagy.

Ma Xiuquan X Jin Mingzhi M Cai Yu Y Xia Hongguang H Long Kai K Liu Junli J Yu Qiang Q Yuan Junying J

Chemistry & biology 20111101 11

Autophagy is a cellular lysosome-dependent catabolic mechanism mediating the turnover of intracellular organelles and long-lived proteins. We show that antimycin A, a known inhibitor of mETC complex III, can inhibit autophagy. A structural and functional study shows that four close analogs of antimycin A that have no effect on mitochondria inhibition also do not inhibit autophagy, whereas myxothiazol, another mETC complex III inhibitor with unrelated structure to antimycin A, inhibits autophagy. ...[more]

PMID: 22118681

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Inhibition of the mitochondrial electron transport chain by antimycin A in Arabidopsis thaliana

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Inhibition of the mitochondrial electron transport chain by antimycin A in Arabidopsis thaliana

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Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-31 | GSE198348 | GEO

Analysis of gene expression profiles from the liver of rats treated with the mitochondrial electron transport chain complex III inhibitor GSK932121A (50mg/kg) or vehicle control

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2015-01-29 | E-GEOD-65374 | biostudies-arrayexpress

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