Rapid empirical discovery of optimal peptides for targeted proteomics.
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ABSTRACT:
SUBMITTER: Stergachis AB
PROVIDER: S-EPMC3227787 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
Rapid empirical discovery of optimal peptides for targeted proteomics.
Stergachis Andrew B AB MacLean Brendan B Lee Kristen K Stamatoyannopoulos John A JA MacCoss Michael J MJ
Nature methods 20111106 12
We report a method for high-throughput, cost-efficient empirical discovery of optimal proteotypic peptides and fragment ions for targeted proteomics applications using in vitro-synthesized proteins. We demonstrate the approach using human transcription factors, which are typically difficult, low-abundance targets and empirically derived proteotypic peptides for 98% of the target proteins. We show that targeted proteomic assays developed using our approach facilitate robust in vivo quantification ...[more]
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