Cloning of the heat shock protein 90 and 70 genes from the beet armyworm, Spodoptera exigua, and expression characteristics in relation to thermal stress and development.

ABSTRACT: Two full-length cDNAs of heat shock protein (HSP) genes (Se-hsp90 and Se-hsp70) were cloned from the beet armyworm, Spodoptera exigua, and their expression was investigated in relation to cold shock, heat shock, and development. The open reading frames of Se-hsp90 and Se-hsp70 are 2,154 and 2,004 bp in length, encoding polypeptides of 717 and 667 amino acids with a molecular mass of 82.6 and 72.5 kDa, respectively. Both genes showed high similarity to their counterparts in other species. Transcriptional expression profiles revealed that both genes were significantly up-regulated under thermal stress. However, the temperature at which expression level became significantly higher than that of controls varied between genes. Intensity of response to temperature was more intense for Se-hsp70 th

SUBMITTER: Jiang X 

PROVIDER: S-EPMC3227854 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature