Ontology highlight
ABSTRACT:
SUBMITTER: Mazzoni EO
PROVIDER: S-EPMC3228994 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Mazzoni Esteban O EO Mahony Shaun S Iacovino Michelina M Morrison Carolyn A CA Mountoufaris George G Closser Michael M Whyte Warren A WA Young Richard A RA Kyba Michael M Gifford David K DK Wichterle Hynek H
Nature methods 20111113 12
The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors ...[more]