Unknown

Dataset Information

0

Embryonic stem cell-based mapping of developmental transcriptional programs.


ABSTRACT: The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.

SUBMITTER: Mazzoni EO 

PROVIDER: S-EPMC3228994 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications


The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors  ...[more]

Similar Datasets

2011-11-12 | E-GEOD-30882 | biostudies-arrayexpress
2011-11-12 | GSE30882 | GEO
| S-EPMC2954693 | biostudies-literature
| S-EPMC3189708 | biostudies-literature
| S-EPMC2743928 | biostudies-literature
| S-EPMC3334447 | biostudies-other
| S-EPMC1570179 | biostudies-literature
| S-EPMC2913321 | biostudies-other
| S-EPMC3548781 | biostudies-literature
| S-EPMC2566604 | biostudies-literature