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Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.

ABSTRACT: BACKGROUND:Thyroid adenoma associated (THADA) has been identified as the target gene affected by chromosome 2p21 translocations in thyroid adenomas, but the role of THADA in the thyroid is still elusive. The aim of this study was to quantify THADA gene expression in normal tissues and in thyroid hyper- and neoplasias, using real-time PCR. METHODS:For the analysis THADA and 18S rRNA gene expression assays were performed on 34 normal tissue samples, including thyroid, salivary gland, heart, endometrium, myometrium, lung, blood, and adipose tissue as well as on 85 thyroid hyper- and neoplasias, including three adenomas with a 2p21 translocation. In addition, NIS (sodium-iodide symporter) gene expression was measured on 34 of the pathological thyroid samples. RESULTS:Results illustrated that THADA expression in normal thyroid tissue was significantly higher (p < 0.0001, exact Wilcoxon test) than in the other tissues. Significant differences were also found between non-malignant pathological thyroid samples (goiters and adenomas) and malignant tumors (p < 0.001, Wilcoxon test, t approximation), anaplastic carcinomas (ATCs) and all other samples and also between ATCs and all other malignant tumors (p < 0.05, Wilcoxon test, t approximation). Furthermore, in thyroid tumors THADA mRNA expression was found to be inversely correlated with HMGA2 mRNA. HMGA2 expression was recently identified as a marker revealing malignant transformation of thyroid follicular tumors. A correlation between THADA and NIS has also been found in thyroid normal tissue and malignant tumors. CONCLUSIONS:The results suggest THADA being a marker of dedifferentiation of thyroid tissue.

SUBMITTER: Kloth L

PROVIDER: S-EPMC3229435 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.

Kloth Lars L Belge Gazanfer G Burchardt Käte K Loeschke Siegfried S Wosniok Werner W Fu Xin X Nimzyk Rolf R Mohamed Salah A SA Drieschner Norbert N Rippe Volkhard V Bullerdiek Jörn J

BMC clinical pathology 20111104

<h4>Background</h4>Thyroid adenoma associated (THADA) has been identified as the target gene affected by chromosome 2p21 translocations in thyroid adenomas, but the role of THADA in the thyroid is still elusive. The aim of this study was to quantify THADA gene expression in normal tissues and in thyroid hyper- and neoplasias, using real-time PCR.<h4>Methods</h4>For the analysis THADA and 18S rRNA gene expression assays were performed on 34 normal tissue samples, including thyroid, salivary gland ...[more]

PMID: 22050638

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Project description:To identify differences in gene expression profiles of infected cells between thyroid carcinoma (C), thyroid adenoma (A) and normal thyroid (N) epithelial cells, differentially expressed genes were identified using three pairwise comparisons with the GEO2R online tool. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to classify them at the functional level. The most significant cluster in the N vs. A pairwise comparison had four hub genes: Insulin-like growth factor 2, Von Willebrand factor (VWF), multimerin 1 (MMRN1) and complement factor D (CFD). In N vs. C, the most significant cluster had 19 genes: IGF2, early growth response 2, transcription factor 3, KIT proto?oncogene receptor tyrosine kinase, SMAD family member 9, MLLT3 super elongation complex subunit, runt related transcription factor 1, CFD, actinin ? 1, SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4, JunD proto?oncogene AP?1 transcription factor subunit, serum response factor (SRF), FosB proto?oncogene, AP?1 transcription factor subunit, connective tissue growth factor (CTGF), SRC proto?oncogene, non?receptor tyrosine kinase, MMRN1, SRY?box 9, early growth response 3 and ETS variant 4. In A vs. C, the most significant cluster had 14 genes: BCL2-like 1, galectin 3, MCL1 BCL2 family apoptosis regulator, DNA damage inducible transcript 3, BCL2 apoptosis regulator, CTGF, matrix metallopeptidase 7, early growth response 1, kinase insert domain receptor, TIMP metallopeptidase inhibitor 1, apolipoprotein E, VWF, cyclin D1 and placental growth factor. Histological evidence was presented to confirm the makeup of the hubs prior to logistic regression analysis to differentiate benign and malignant neoplasms. The results of the present study may aid in the search for novel potential biomarkers for the differential diagnosis, prognosis and development of drug targets of thyroid neoplasm.

Dataset Information

Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.

Publications

Decrease in thyroid adenoma associated (THADA) expression is a marker of dedifferentiation of thyroid tissue.

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