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Measurement of warfarin in the oral fluid of patients undergoing anticoagulant oral therapy.


ABSTRACT:

Background

Patients on warfarin therapy undergo invasive and expensive checks for the coagulability of their blood. No information on coagulation levels is currently available between two controls.

Methodology

A method was developed to determine warfarin in oral fluid by HPLC and fluorimetric detection. The chromatographic separation was performed at room temperature on a C-18 reversed-phase column, 65% PBS and 35% methanol mobile phase, flow rate 0.7 mL/min, injection volume 25 µL, excitation wavelength 310 nm, emission wavelength 400 nm.

Findings

The method was free from interference and matrix effect, linear in the range 0.2-100 ng/mL, with a detection limit of 0.2 ng/mL. Its coefficient of variation was <3% for intra-day measurements and <5% for inter-day measurements. The average concentration of warfarin in the oral fluid of 50 patients was 2.5±1.6 ng/mL (range 0.8-7.6 ng/mL). Dosage was not correlated to INR (r?=?-0.03, p?=?0.85) but positively correlated to warfarin concentration in the oral fluid (r?=?0.39, p?=?0.006). The correlation between warfarin concentration and pH in the oral fluid (r?=?0.37, p?=?0.009) confirmed the importance of pH in regulating the drug transfer from blood. A correlation between warfarin concentration in the oral fluid and INR was only found in samples with pH values ?7.2 (r?=?0.84, p?=?0.004).

Conclusions

Warfarin diffuses from blood to oral fluid. The method allows to measure its concentration in this matrix and to analyze correlations with INR and other parameters.

SUBMITTER: Ghimenti S 

PROVIDER: S-EPMC3229510 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Publications

Measurement of warfarin in the oral fluid of patients undergoing anticoagulant oral therapy.

Ghimenti Silvia S   Lomonaco Tommaso T   Onor Massimo M   Murgia Laura L   Paolicchi Aldo A   Fuoco Roger R   Ruocco Lucia L   Pellegrini Giovanni G   Trivella Maria Giovanna MG   Di Francesco Fabio F  

PloS one 20111202 12


<h4>Background</h4>Patients on warfarin therapy undergo invasive and expensive checks for the coagulability of their blood. No information on coagulation levels is currently available between two controls.<h4>Methodology</h4>A method was developed to determine warfarin in oral fluid by HPLC and fluorimetric detection. The chromatographic separation was performed at room temperature on a C-18 reversed-phase column, 65% PBS and 35% methanol mobile phase, flow rate 0.7 mL/min, injection volume 25 µ  ...[more]

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