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Global DNA demethylation during mouse erythropoiesis in vivo.

ABSTRACT: In the mammalian genome, 5'-CpG-3' dinucleotides are frequently methylated, correlating with transcriptional silencing. Genome-wide demethylation is thought to occur only twice during development, in primordial germ cells and in the pre-implantation embryo. These demethylation events are followed by de novo methylation, setting up a pattern inherited throughout development and modified only at tissue-specific loci. We studied DNA methylation in differentiating mouse erythroblasts in vivo by using genomic-scale reduced representation bisulfite sequencing (RRBS). Demethylation at the erythroid-specific ?-globin locus was coincident with global DNA demethylation at most genomic elements. Global demethylation was continuous throughout differentiation and required rapid DNA replication. Hence, DNA demethylation can occur globally during somatic cell differentiation, providing an experimental model for its study in development and disease.

SUBMITTER: Shearstone JR

PROVIDER: S-EPMC3230325 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Global DNA demethylation during mouse erythropoiesis in vivo.

Shearstone Jeffrey R JR Pop Ramona R Bock Christoph C Boyle Patrick P Meissner Alexander A Socolovsky Merav M

Science (New York, N.Y.) 20111101 6057

In the mammalian genome, 5'-CpG-3' dinucleotides are frequently methylated, correlating with transcriptional silencing. Genome-wide demethylation is thought to occur only twice during development, in primordial germ cells and in the pre-implantation embryo. These demethylation events are followed by de novo methylation, setting up a pattern inherited throughout development and modified only at tissue-specific loci. We studied DNA methylation in differentiating mouse erythroblasts in vivo by usin ...[more]

PMID: 22076376

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