Unknown

Dataset Information

0

A 1.5 A resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase.


ABSTRACT: A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R(free) of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 Å and a sequence identity of 53%. This structural similarity is significant considering that C. bescii is a hyperthermophile and Bacillus sp. is a mesophile.

SUBMITTER: Alahuhta M 

PROVIDER: S-EPMC3232124 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase.

Alahuhta Markus M   Chandrayan Puja P   Kataeva Irina I   Adams Michael W W MW   Adams Michael W W MW   Himmel Michael E ME   Lunin Vladimir V VV  

Acta crystallographica. Section F, Structural biology and crystallization communications 20111125 Pt 12


A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R(free) of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 Å and a sequence identity of 53%. This structural  ...[more]

Similar Datasets

| S-EPMC4556314 | biostudies-literature
| S-EPMC7071700 | biostudies-literature
| S-EPMC6685990 | biostudies-literature
2017-11-28 | GSE107393 | GEO
| S-EPMC4066518 | biostudies-other
| PRJNA396455 | ENA