Ontology highlight
ABSTRACT:
SUBMITTER: Atkinson SC
PROVIDER: S-EPMC3232133 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Atkinson Sarah C SC Dogovski Con C Newman Janet J Dobson Renwick C J RC Perugini Matthew A MA
Acta crystallographica. Section F, Structural biology and crystallization communications 20111125 Pt 12
Dihydrodipicolinate synthase (DHDPS) catalyses the first committed step of the lysine-biosynthesis pathway in bacteria, plants and some fungi. This study describes the cloning, expression, purification and crystallization of DHDPS from the grapevine Vitis vinifera (Vv-DHDPS). Following in-drop cleavage of the hexahistidine tag, cocrystals of Vv-DHDPS with the substrate pyruvate were grown in 0.1 M Bis-Tris propane pH 8.2, 0.2 M sodium bromide, 20%(w/v) PEG 3350. X-ray diffraction data in space g ...[more]