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Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans.

ABSTRACT: Streptococcus mutans dextranase hydrolyzes the internal ?-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2(1), with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, ? = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å(3) Da(-1); assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å(3) Da(-1).

SUBMITTER: Suzuki N

PROVIDER: S-EPMC3232134 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans.

Suzuki Nobuhiro N Kim Young Min YM Fujimoto Zui Z Momma Mitsuru M Kang Hee Kwon HK Funane Kazumi K Okuyama Masayuki M Mori Haruhide H Kimura Atsuo A

Acta crystallographica. Section F, Structural biology and crystallization communications 20111125 Pt 12

Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2(1), with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficie ...[more]

PMID: 22139161

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