Ontology highlight
ABSTRACT:
SUBMITTER: McGlinchey RP
PROVIDER: S-EPMC3232329 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
McGlinchey Ryan P RP Gruschus James M JM Nagy Attila A Lee Jennifer C JC
Biochemistry 20111117 49
Pmel17 is a human amyloid involved in melanin synthesis. A fragment of Pmel17, the repeat domain (RPT) rich in glutamic acids, forms amyloid only at mildly acidic pH. Unlike pathological amyloids, these fibrils dissolve at neutral pH, supporting a reversible aggregation-disaggregation process. Here, we study RPT dissolution using atomic force microscopy and solution-state nuclear magnetic resonance spectroscopy. Our results reveal asymmetric fibril disassembly proceeding in the absence of interm ...[more]