Unknown

Dataset Information

0

Efg1 Controls caspofungin-induced cell aggregation of Candida albicans through the adhesin Als1.


ABSTRACT: Echinocandin drugs such as caspofungin (CASP), micafungin, and anidulafungin inhibit fungal cell wall biogenesis by blocking Fks1-mediated ?-glucan deposition into the cell surface. Candins have become suitable drugs to treat life-threatening diseases caused by several fungal species, including Candida albicans, that are pathogenic for humans. Here, we present the discovery of a novel CASP-induced flocculation phenotype of C. albicans, which formed large cell aggregates in the presence of CASP. High concentrations of sugars such as mannose or glucose inhibit CASP-induced flocculation and improve survival of C. albicans cells exposed to CASP. Notably, exposure of C. albicans cells to CASP triggers Efg1-dependent expression of the adhesin ALS1 and induces invasive growth on agar plates. Indeed, cells lacking either Efg1 or Als1 show strongly diminished CASP-induced flocculation, and the absence of Efg1 leads to marked CASP hypersensitivity. On the other hand, CASP-induced invasive growth is enhanced in cells lacking Efg1. Hence, CASP stress drives an Efg1-dependent response, indicating that this multifunctional transcriptional regulator, which is otherwise involved in filamentation, white-to-opaque switching, and virulence, also modulates cell wall remodeling upon CASP challenge. Taken together, our data suggest that CASP-induced cell wall damage activates Efg1 in parallel with the known cell integrity stress signaling pathway to coordinate cell wall remodeling.

SUBMITTER: Gregori C 

PROVIDER: S-EPMC3232723 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Efg1 Controls caspofungin-induced cell aggregation of Candida albicans through the adhesin Als1.

Gregori Christa C   Glaser Walter W   Frohner Ingrid E IE   Reinoso-Martín Cristina C   Rupp Steffen S   Schüller Christoph C   Kuchler Karl K  

Eukaryotic cell 20111028 12


Echinocandin drugs such as caspofungin (CASP), micafungin, and anidulafungin inhibit fungal cell wall biogenesis by blocking Fks1-mediated β-glucan deposition into the cell surface. Candins have become suitable drugs to treat life-threatening diseases caused by several fungal species, including Candida albicans, that are pathogenic for humans. Here, we present the discovery of a novel CASP-induced flocculation phenotype of C. albicans, which formed large cell aggregates in the presence of CASP.  ...[more]

Similar Datasets

| S-EPMC7848995 | biostudies-literature
| S-EPMC3564254 | biostudies-literature
| S-EPMC7269472 | biostudies-literature
| S-EPMC2394972 | biostudies-literature
| S-EPMC2687269 | biostudies-literature
| S-EPMC5988767 | biostudies-literature
| S-EPMC4664712 | biostudies-literature
| S-EPMC3851762 | biostudies-literature
| S-EPMC7931687 | biostudies-literature
| S-EPMC6838528 | biostudies-literature