Ontology highlight
ABSTRACT:
SUBMITTER: Shen Y
PROVIDER: S-EPMC3233426 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Shen Yanfei Y Wang Fanghua F Lan Dongming D Liu Yuanyuan Y Yang Bo B Wang Yonghua Y
International journal of molecular sciences 20111107 11
Experiments were carried out to investigate the effects of various factors on the activity and conformation of recombinant leucine aminopeptidase of Bacillus kaustophilus CCRC 11223 (BkLAP) and potential utilization of BkLAP in the hydrolysis of anchovy protein. Optimal temperature and pH of BkLAP were 70 °C and 8.0 in potassium-phosphate buffer, respectively, and the activity was strongly stimulated by Ni(2+), followed by Mn(2+) and Co(2+). Conformational studies via circular dichroism spectros ...[more]