Dataset Information
Dendrimer-based fluorescent indicators: in vitro and in vivo applications.
Ontology highlight
ABSTRACT: Background
The development of fluorescent proteins and synthetic molecules whose fluorescence properties are controlled by the environment makes it possible to monitor physiological and pathological events in living systems with minimal perturbation. A large number of small organic dyes are available and routinely used to measure biologically relevant parameters. Unfortunately their application is hindered by a number of limitations stemming from the use of these small molecules in the biological environment.Principal findings
We present a novel dendrimer-based architecture leading to multifunctional sensing elements that can overcome many of these problems. Applications in vitro, in living cells and in vivo are reported. In particular, we image for the first time extracellular pH in the brain in a mouse epilepsy model.Conclusion
We believe that the proposed architecture can represent a useful and novel tool in fluorescence imaging that can be widely applied in conjunction with a broad range of sensing dyes and experimental setups.
SUBMITTER: Albertazzi L
PROVIDER: S-EPMC3233578 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Dendrimer-based fluorescent indicators: in vitro and in vivo applications.
PloS one 20111207 12
<h4>Background</h4>The development of fluorescent proteins and synthetic molecules whose fluorescence properties are controlled by the environment makes it possible to monitor physiological and pathological events in living systems with minimal perturbation. A large number of small organic dyes are available and routinely used to measure biologically relevant parameters. Unfortunately their application is hindered by a number of limitations stemming from the use of these small molecules in the b ...[more]