Bcl-2-interacting mediator of cell death influences autoantigen-driven deletion and TCR revision.

ABSTRACT: Peripheral CD4(+)V?5(+) T cells are tolerized to an endogenous mouse mammary tumor virus superantigen either by deletion or TCR revision. Through TCR revision, RAG reexpression mediates extrathymic TCR? rearrangement and results in a population of postrevision CD4(+)V?5(-) T cells expressing revised TCR? chains. We have hypothesized that cell death pathways regulate the selection of cells undergoing TCR revision to ensure the safety and utility of the postrevision population. In this study, we investigate the role of Bcl-2-interacting mediator of cell death (Bim)-mediated cell death in autoantigen-driven deletion and TCR revision. Bim deficiency and Bcl-2 overexpression in V?5 transgenic (Tg) mice both impair peripheral deletion. V?5 Tg Bim-deficient and Bcl-2 Tg mice exhibit an elevated frequency of CD4(+) T cells expressing both the transgene-encoded V?5 chain and a revised TCR? chain. We now show that these dual-TCR-expressing cells are TCR revision intermediates and that the population of RAG-expressing, revising CD4(+) T cells is increased in Bim-deficient V?5 Tg mice. These findings support a role for Bim and Bcl-2 in regulating the balance of survival versus apoptosis in peripheral T cells undergoing RAG-dependent TCR rearrangements during TCR revision, thereby ensuring the utility of the postrevision repertoire.

SUBMITTER: Hale JS 

PROVIDER: S-EPMC3233758 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature