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A public genome-scale lentiviral expression library of human ORFs.

ABSTRACT: Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

SUBMITTER: Yang X

PROVIDER: S-EPMC3234135 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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A public genome-scale lentiviral expression library of human ORFs.

Yang Xiaoping X Boehm Jesse S JS Yang Xinping X Salehi-Ashtiani Kourosh K Hao Tong T Shen Yun Y Lubonja Rakela R Thomas Sapana R SR Alkan Ozan O Bhimdi Tashfeen T Green Thomas M TM Johannessen Cory M CM Silver Serena J SJ Nguyen Cindy C Murray Ryan R RR Hieronymus Haley H Balcha Dawit D Fan Changyu C Lin Chenwei C Ghamsari Lila L Vidal Marc M Hahn William C WC Hill David E DE Root David E DE

Nature methods 20110626 8

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput scre ...[more]

PMID: 21706014

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