Ontology highlight
ABSTRACT:
SUBMITTER: Wilke S
PROVIDER: S-EPMC3235087 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Wilke Sonja S Groebe Lothar L Maffenbeier Vitali V Jäger Volker V Gossen Manfred M Josewski Jörn J Duda Agathe A Polle Lilia L Owens Raymond J RJ Wirth Dagmar D Heinz Dirk W DW van den Heuvel Joop J Büssow Konrad K
PloS one 20111209 12
Studying the biophysical characteristics of glycosylated proteins and solving their three-dimensional structures requires homogeneous recombinant protein of high quality.We introduce here a new approach to produce glycoproteins in homogenous form with the well-established, glycosylation mutant CHO Lec3.2.8.1 cells. Using preparative cell sorting, stable, high-expressing GFP 'master' cell lines were generated that can be converted fast and reliably by targeted integration via Flp recombinase-medi ...[more]