Identifying hosts of families of viruses: a machine learning approach.
Ontology highlight
ABSTRACT:
SUBMITTER: Raj A
PROVIDER: S-EPMC3235098 | biostudies-literature |
REPOSITORIES: biostudies-literature
Similar Datasets
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2023-06-01 | GSE193400 | GEO
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Project description:Remote sensing techniques are now commonly applied to map and monitor urban land uses to measure growth and to assist with development and planning. Recent work in this area has highlighted the use of textures and other spatial features that can be measured in very high spatial resolution imagery. Far less attention has been given to using geospatial vector data (i.e. points, lines, polygons) to map land uses. This paper presents an approach to distinguish residential settlement types (regular vs. irregular) using an existing database of settlement points locating structures. Nine data features describing the density, distance, angles, and spacing of the settlement points are calculated at multiple spatial scales. These data are analysed alone and with five common remote sensing measures on elevation, slope, vegetation, and nighttime lights in a supervised machine learning approach to classify land use areas. The method was tested in seven provinces of Afghanistan (Balkh, Helmand, Herat, Kabul, Kandahar, Kunduz, Nangarhar). Overall accuracy ranged from 78% in Kandahar to 90% in Nangarhar. This research demonstrates the potential to accurately map land uses from even the simplest representation of structures.
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Project description:BackgroundThis paper exploits recent developments in topological data analysis to present a pipeline for clustering based on Mapper, an algorithm that reduces complex data into a one-dimensional graph.ResultsWe present a pipeline to identify and summarise clusters based on statistically significant topological features from a point cloud using Mapper.ConclusionsKey strengths of this pipeline include the integration of prior knowledge to inform the clustering process and the selection of optimal clusters; the use of the bootstrap to restrict the search to robust topological features; the use of machine learning to inspect clusters; and the ability to incorporate mixed data types. Our pipeline can be downloaded under the GNU GPLv3 license at https://github.com/kcl-bhi/mapper-pipeline .
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Project description:Transcriptional enhancers integrate the contributions of multiple classes of transcription factors (TFs) to orchestrate the myriad spatio-temporal gene expression programs that occur during development. A molecular understanding of enhancers with similar activities requires the identification of both their unique and their shared sequence features. To address this problem, we combined phylogenetic profiling with a DNA-based enhancer sequence classifier that analyzes the TF binding sites (TFBSs) governing the transcription of a co-expressed gene set. We first assembled a small number of enhancers that are active in Drosophila melanogaster muscle founder cells (FCs) and other mesodermal cell types. Using phylogenetic profiling, we increased the number of enhancers by incorporating orthologous but divergent sequences from other Drosophila species. Functional assays revealed that the diverged enhancer orthologs were active in largely similar patterns as their D. melanogaster counterparts, although there was extensive evolutionary shuffling of known TFBSs. We then built and trained a classifier using this enhancer set and identified additional related enhancers based on the presence or absence of known and putative TFBSs. Predicted FC enhancers were over-represented in proximity to known FC genes; and many of the TFBSs learned by the classifier were found to be critical for enhancer activity, including POU homeodomain, Myb, Ets, Forkhead, and T-box motifs. Empirical testing also revealed that the T-box TF encoded by org-1 is a previously uncharacterized regulator of muscle cell identity. Finally, we found extensive diversity in the composition of TFBSs within known FC enhancers, suggesting that motif combinatorics plays an essential role in the cellular specificity exhibited by such enhancers. In summary, machine learning combined with evolutionary sequence analysis is useful for recognizing novel TFBSs and for facilitating the identification of cognate TFs that coordinate cell type-specific developmental gene expression patterns.
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