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Ribosomal DNA and morphological analysis of Dactylogyrus species from freshwater fishes of India.

ABSTRACT: Dactylogyrus species (Dactylogyrideae: Monogenea) are a group of monogenean gill parasites these parasites are highly host specific to freshwater fish of family Cyprinidae. A method is presented for the isolation and morphometric analysis of hamuli marginal hooks and bars form individual dactylogrids monogenoidea using phase contrast microcopy and camera lucida drawing, while simultaneously processing for rDNA analysis using the polymerase chain reaction. The identification and taxonomy of the worms in question was done by the morphometrical methods and rDNA analysis. ITS-1 regions of the rDNA gene of worms were amplified, sequenced and then the discrimination was recorded at the species level. The studied segments were compared with the published data on such segments of other Dactylogyrids species and were found to have significant relationship in the genes and spacer regions of the rDNA.

SUBMITTER: Sharma P

PROVIDER: S-EPMC3235379 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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Ribosomal DNA and morphological analysis of Dactylogyrus species from freshwater fishes of India.

Sharma Prapunjika P Agarwal Nirupama N Kumar Sudhir S

Journal of parasitic diseases : official organ of the Indian Society for Parasitology 20110811 2

Dactylogyrus species (Dactylogyrideae: Monogenea) are a group of monogenean gill parasites these parasites are highly host specific to freshwater fish of family Cyprinidae. A method is presented for the isolation and morphometric analysis of hamuli marginal hooks and bars form individual dactylogrids monogenoidea using phase contrast microcopy and camera lucida drawing, while simultaneously processing for rDNA analysis using the polymerase chain reaction. The identification and taxonomy of the w ...[more]

PMID: 23024507

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Project description:BackgroundDNA barcoding aims to provide an efficient method for species-level identifications using an array of species specific molecular tags derived from the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene. The efficiency of the method hinges on the degree of sequence divergence among species and species-level identifications are relatively straightforward when the average genetic distance among individuals within a species does not exceed the average genetic distance between sister species. Fishes constitute a highly diverse group of vertebrates that exhibit deep phenotypic changes during development. In this context, the identification of fish species is challenging and DNA barcoding provide new perspectives in ecology and systematics of fishes. Here we examined the degree to which DNA barcoding discriminate freshwater fish species from the well-known Canadian fauna, which currently encompasses nearly 200 species, some which are of high economic value like salmons and sturgeons.Methodology/principal findingsWe bi-directionally sequenced the standard 652 bp "barcode" region of COI for 1360 individuals belonging to 190 of the 203 Canadian freshwater fish species (95%). Most species were represented by multiple individuals (7.6 on average), the majority of which were retained as voucher specimens. The average genetic distance was 27 fold higher between species than within species, as K2P distance estimates averaged 8.3% among congeners and only 0.3% among concpecifics. However, shared polymorphism between sister-species was detected in 15 species (8% of the cases). The distribution of K2P distance between individuals and species overlapped and identifications were only possible to species group using DNA barcodes in these cases. Conversely, deep hidden genetic divergence was revealed within two species, suggesting the presence of cryptic species.Conclusions/significanceThe present study evidenced that freshwater fish species can be efficiently identified through the use of DNA barcoding, especially the species complex of small-sized species, and that the present COI library can be used for subsequent applications in ecology and systematics.

Dataset Information

Ribosomal DNA and morphological analysis of Dactylogyrus species from freshwater fishes of India.

Publications

Ribosomal DNA and morphological analysis of Dactylogyrus species from freshwater fishes of India.

Similar Datasets

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