An Escherichia coli-based bioengineering strategy to study streptolysin S biosynthesis.
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ABSTRACT:
SUBMITTER: Markley AL
PROVIDER: S-EPMC3240940 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
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An Escherichia coli-based bioengineering strategy to study streptolysin S biosynthesis.
Markley Andrew L AL Jensen Emily R ER Lee Shaun W SW
Analytical biochemistry 20110922 2
Group A Streptococcus pyogenes (GAS) is a leading human pathogen that produces a powerful cytolytic bacteriocin known as streptolysin S (SLS). We have developed a bioengineering strategy to successfully reconstitute SLS activity using heterologous expression in laboratory strains of Escherichia coli. Our E. coli-based heterologous expression system will allow more detailed studies into the biosynthesis of other bacteriocin compounds and the production of these natural products in much greater yi ...[more]
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