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Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.

ABSTRACT: A quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.

SUBMITTER: Showmaker K

PROVIDER: S-EPMC3241691 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.

Showmaker Kurt K Lawrence Gary W GW Lu Shien S Balbalian Clarissa C Klink Vincent P VP

PloS one 20111216 12

A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil. ...[more]

PMID: 22194958

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