Ontology highlight
ABSTRACT:
SUBMITTER: Fonseca DM
PROVIDER: S-EPMC3241966 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Fonseca Dina M DM Okada Kenli K Kramer Laura D LD
Molecular ecology resources 20090122 3
We characterized the first microsatellite loci in the white-dotted mosquito, Culex restuans, a critical early spring West Nile virus vector. An enrichment protocol yielded 960 positive clones of which we sequenced 300. We designed primers to amplify 29 unique di-, tri- and tetranucleotide microsatellites and chose 17 that amplified consistently across populations and were polymorphic. We developed three multiplex primer combinations for all 17 loci. A survey of 44 individuals revealed two to 20 ...[more]