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Molecular evidence for a natural primary triple hybrid in plants revealed from direct sequencing.

ABSTRACT: BACKGROUND AND AIMS:Molecular evidence for natural primary hybrids composed of three different plant species is very rarely reported. An investigation was therefore carried out into the origin and a possible scenario for the rise of a sterile plant clone showing a combination of diagnostic morphological features of three separate, well-defined Potamogeton species. METHODS:The combination of sequences from maternally inherited cytoplasmic (rpl20-rps12) and biparentally inherited nuclear ribosomal DNA (ITS) was used to identify the exact identity of the putative triple hybrid. KEY RESULTS:Direct sequencing showed ITS variants of three parental taxa, P. gramineus, P. lucens and P. perfoliatus, whereas chloroplast DNA identified P. perfoliatus as the female parent. A scenario for the rise of the triple hybrid through a fertile binary hybrid P. gramineus x P. lucens crossed with P. perfoliatus is described. CONCLUSIONS:Even though the triple hybrid is sterile, it possesses an efficient strategy for its existence and became locally successful even in the parental environment, perhaps as a result of heterosis. The population investigated is the only one known of this hybrid, P. x torssanderi, worldwide. Isozyme analysis indicated the colony to be genetically uniform. The plants studied represented a single clone that seems to have persisted at this site for a long time.

SUBMITTER: Kaplan Z

PROVIDER: S-EPMC3243585 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature

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Molecular evidence for a natural primary triple hybrid in plants revealed from direct sequencing.

Kaplan Zdenek Z Fehrer Judith J

Annals of botany 20070503 6

<h4>Background and aims</h4>Molecular evidence for natural primary hybrids composed of three different plant species is very rarely reported. An investigation was therefore carried out into the origin and a possible scenario for the rise of a sterile plant clone showing a combination of diagnostic morphological features of three separate, well-defined Potamogeton species.<h4>Methods</h4>The combination of sequences from maternally inherited cytoplasmic (rpl20-rps12) and biparentally inherited nu ...[more]

PMID: 17478544

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Molecular evidence for a natural primary triple hybrid in plants revealed from direct sequencing.

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Molecular evidence for a natural primary triple hybrid in plants revealed from direct sequencing.

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