Unknown

Dataset Information

0

Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis.


ABSTRACT: The 90-kDa isoform of the lipid kinase PIP kinase Type I ? (PIPKI?) localizes to focal adhesions (FAs), where it provides a local source of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)). Although PtdIns(4,5)P(2) regulates the function of several FA-associated molecules, the role of the FA-specific pool of PtdIns(4,5)P(2) is not known. We report that the genetic ablation of PIPKI? specifically from FAs results in defective integrin-mediated adhesion and force coupling. Adhesion defects in cells deficient in FAPtdIns(4,5)P(2) synthesis are corrected within minutes while integrin-actin force coupling remains defective over a longer period. Talin and vinculin, but not kindlin, are less efficiently recruited to new adhesions in these cells. These data demonstrate that the specific depletion of PtdIns(4,5)P(2) from FAs temporally separates integrin-ligand binding from integrin-actin force coupling by regulating talin and vinculin recruitment. Furthermore, it suggests that force coupling relies heavily on locally generated PtdIns(4,5)P(2) rather than bulk membrane PtdIns(4,5)P(2).

SUBMITTER: Legate KR 

PROVIDER: S-EPMC3243596 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis.

Legate Kyle R KR   Takahashi Seiichiro S   Bonakdar Navid N   Fabry Ben B   Boettiger David D   Zent Roy R   Fässler Reinhard R  

The EMBO journal 20111101 22


The 90-kDa isoform of the lipid kinase PIP kinase Type I γ (PIPKIγ) localizes to focal adhesions (FAs), where it provides a local source of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)). Although PtdIns(4,5)P(2) regulates the function of several FA-associated molecules, the role of the FA-specific pool of PtdIns(4,5)P(2) is not known. We report that the genetic ablation of PIPKIγ specifically from FAs results in defective integrin-mediated adhesion and force coupling. Adhesion defects  ...[more]

Similar Datasets

| S-EPMC8906353 | biostudies-literature
| S-EPMC6124568 | biostudies-literature
| S-EPMC3304020 | biostudies-literature
| S-EPMC6059376 | biostudies-literature
| S-EPMC1462986 | biostudies-literature
| S-EPMC3138702 | biostudies-literature
| S-EPMC4582195 | biostudies-literature
| S-EPMC3533393 | biostudies-literature
| S-EPMC2749144 | biostudies-literature
| S-EPMC3625291 | biostudies-literature