Ontology highlight
ABSTRACT:
SUBMITTER: Soliakov A
PROVIDER: S-EPMC3244517 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Soliakov Andrei A Kelly Ian F IF Lakey Jeremy H JH Watkinson Allan A
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 20110922 1
An anthrax sub-unit vaccine, comprising recombinant Protective Antigen (rPA83) and aluminium hydroxide adjuvant (Alhydrogel®) is currently being developed. Here, a series of biophysical techniques have been applied to free and adjuvant bound antigen. Limited proteolysis and fluorescence identified no changes in rPA83 tertiary structure following binding to Alhydrogel and the bound rPA83 retained two structurally important calcium ions. For adsorbed rPA83, differential scanning calorimetry reveal ...[more]