Quantification and monitoring of inflammation in murine inflammatory bowel disease with targeted contrast-enhanced US.

ABSTRACT:

Purpose

To evaluate ultrasonography (US) by using contrast agent microbubbles (MBs) targeted to P-selectin (MB(P-selectin)) to quantify P-selectin expression levels in inflamed tissue and to monitor response to therapy in a murine model of chemically induced inflammatory bowel disease (IBD).

Materials and methods

All procedures in which laboratory animals were used were approved by the institutional administrative panel on laboratory animal care. Binding affinity and specificity of MB(P-selectin) were tested in cell culture experiments under flow shear stress conditions and compared with control MBs (MB(Control)). In vivo binding specificity of MB(P-selectin) to P-selectin was tested in mice with trinitrobenzenesulfonic acid-induced colitis (n = 22) and control mice (n = 10)

SUBMITTER: Deshpande N 

PROVIDER: S-EPMC3244669 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature