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Single cell transcriptomics of neighboring hyphae of Aspergillus niger.

ABSTRACT: Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.

SUBMITTER: de Bekker C

PROVIDER: S-EPMC3245611 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Single cell transcriptomics of neighboring hyphae of Aspergillus niger.

de Bekker Charissa C Bruning Oskar O Jonker Martijs J MJ Breit Timo M TM Wösten Han A B HA

Genome biology 20110804 8

Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories. ...[more]

PMID: 21816052

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