Colorful virus-like particles: fluorescent protein packaging by the Q? capsid.
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ABSTRACT:
SUBMITTER: Rhee JK
PROVIDER: S-EPMC3246388 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
Colorful virus-like particles: fluorescent protein packaging by the Qβ capsid.
Rhee Jin-Kyu JK Hovlid Marisa M Fiedler Jason D JD Brown Steven D SD Manzenrieder Florian F Kitagishi Hiroaki H Nycholat Corwin C Paulson James C JC Finn M G MG
Biomacromolecules 20111013 11
Qβ virus-like particles encapsulating multiple copies of fluorescent proteins were generated in high yields using a modular system enhanced by specific engineered RNA--protein interactions. The resulting particles were structurally indistinguishable from recombinant Qβ alone. The encapsidated proteins were nearly identical in photochemical properties to monomeric analogues, were more stable toward thermal degradation, and were protected from proteolytic cleavage. Residues on the outer capsid sur ...[more]
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