Ontology highlight
ABSTRACT:
SUBMITTER: Underwood JG
PROVIDER: S-EPMC3247016 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Underwood Jason G JG Uzilov Andrew V AV Katzman Sol S Onodera Courtney S CS Mainzer Jacob E JE Mathews David H DH Lowe Todd M TM Salama Sofie R SR Haussler David D
Nature methods 20101107 12
Classical approaches to determine structures of noncoding RNA (ncRNA) probed only one RNA at a time with enzymes and chemicals, using gel electrophoresis to identify reactive positions. To accelerate RNA structure inference, we developed fragmentation sequencing (FragSeq), a high-throughput RNA structure probing method that uses high-throughput RNA sequencing of fragments generated by digestion with nuclease P1, which specifically cleaves single-stranded nucleic acids. In experiments probing the ...[more]