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Improved culture-based isolation of differentiating endothelial progenitor cells from mouse bone marrow mononuclear cells.


ABSTRACT: Numerous endothelial progenitor cell (EPC)-related investigations have been performed in mouse experiments. However, defined characteristics of mouse cultured EPC have not been examined. We focused on fast versus slow adherent cell population in bone marrow mononuclear cells (BMMNCs) in culture and examined their characteristics. After 24 h-culture of BMMNCs, attached (AT) cells and floating (FL) cells were further cultured in endothelial differentiation medium separately. Immunological and molecular analyses exhibited more endothelial-like and less monocyte/macrophage-like characteristics in FL cells compared with AT cells. FL cells formed thick/stable tube and hypoxia or shear stress overload further enhanced these endothelial-like features with increased angiogenic cytokine/growth factor mRNA expressions. Finally, FL cells exhibited therapeutic potential in a mouse myocardial infarction model showing the specific local recruitment to ischemic border zone and tissue preservation. These findings suggest that slow adherent (FL) but not fast attached (AT) BMMNCs in culture are EPC-rich population in mouse.

SUBMITTER: Sekiguchi H 

PROVIDER: S-EPMC3247221 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Improved culture-based isolation of differentiating endothelial progenitor cells from mouse bone marrow mononuclear cells.

Sekiguchi Haruki H   Ii Masaaki M   Jujo Kentaro K   Yokoyama Ayumi A   Hagiwara Nobuhisa N   Asahara Takayuki T  

PloS one 20111228 12


Numerous endothelial progenitor cell (EPC)-related investigations have been performed in mouse experiments. However, defined characteristics of mouse cultured EPC have not been examined. We focused on fast versus slow adherent cell population in bone marrow mononuclear cells (BMMNCs) in culture and examined their characteristics. After 24 h-culture of BMMNCs, attached (AT) cells and floating (FL) cells were further cultured in endothelial differentiation medium separately. Immunological and mole  ...[more]

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